Cytoplasmic linker protein of 170kDa (CLIP-170) is the original member of a group of microtuble binding proteins designated as plus-end-binding proteins(+TIPs).
CLIP-170 binds to the growing plus ends of microtubules and acts as a linker between the dynamic microtubule ends and organelle membranes.
The protein acts as an anticatastrophic factor, promoting microtubule rescue near the cell periphery.
Fluorescently labeled CLIP-170 can be visualized as a comet like streak around the growing ends of microtubules.
CLIP-170 colocalizes with dynactin and dynein at microtubule ends and also at the kinetochore.
Restin, first identified as a marker for Hodgkin and Reed-Sternberg (HRS) cells, is a 160kDa splice variant of the gene that includes a 35 amino acid stretch not present in CLIP-170.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CLIP-170. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1300-1350 of Human CLIP-170.
