The inter-alpha trypsin inhibitor (ITI) family is a group of structurally related plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-H1, ITI-H2 and ITI-H3) and one light chain (Bikunin).
A fourth member of the ITI family, ITI-H4 (also known as I a IH4P) harbors a Pro-rich region (PRR) in its C-terminus.
ITI is a glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 and ITI-H2, and Bikunin.
Bikunin confers the protease-inhibitor function of ITI.
The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins), bind covalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization.
ITI-H1 contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
Although ITI-H1, ITI-H3 and Bikunin have antitumoral and antimetastatic properties in the cell, they are also associated with malignant transformation of lung tissue.
ITI-H1 and ITI-H2 are associated with calcium oxalate stone formation in kidney and urine.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Cleaved-ITI. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 650-700 of Human ITI-H1.
