The claudin superfamily consists of many structurally related proteins in humans.
These proteins are important structural and functional components of tight junctions in paracellular transport.
Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues.
Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and junction adhesion molecule.
Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands.
Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions.
Claudin-6 is expressed in differentiated F9 cells that resemble tight junction-bearing viceral endoderm resulting from stimulation with retinoc acid and mediated by RXRalpha and RARγ.
Claudin-6 is absent in mouse brain and lung.
The human claudin-6 gene maps to chromosome 16p13.3.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Claudin-6. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 100-150 of Human Claudin-6.
