The Claudin superfamily consists of many structurally related proteins in humans.
These proteins are important structural and functional components of tight junctions in paracellular transport.
Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues.
Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands.
Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions.
Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas.
Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrone, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Claudin 5. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa171-220 of human Claudin 5.
