Platelets regulate the function of Integrin alpha2b/beta3 (GPIIb/IIIa), the platelet Fibrinogen receptor, which is involved in the binding of proteins to integrin cytoplasmic domains.
A novel protein, CIB, for calcium- and integrin-binding protein (also designated as Kip for kinase interacting protein, SIP2-28 and DNA-PKcs interacting protein), binds specifically at the cytoplasmic domain of alpha2b by a number of positively charged residues in its binding site.
Binding of CIB to the alpha2b is affected by fluctuations in the intracellular calcium concentration.
In aggregated platelets, endogenous CIB and alpha2b/beta3 translocate to the Triton X-100-insoluble cytoskeleton, demonstrating that the cellular localization of CIB is regulated.
CIB also binds to DNA-PKcs, which is a nu-clear protein serine/threonine kinase that plays a role in the DNA repair and recombination process of lymphoid development.
Fnk also binds to the CIB, suggesting that CIB may be a regulatory subunit of polo-like kinases.
CIB shows significant homology to calcineurin B and calmodulin, and its mRNA levels are ubiquitously expressed in various human tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CIB1. Species Crossreactivity: Human, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to residues in Human CIB1.
