Carbohydrate sulfotransferase 1 (CHST1), also referred to as KSGal6ST or KSST, is a keratan sulfate sulfotransferase.
It is predominantly expressed in brain and skeletal muscle and localizes to the trans-Golgi network.
CHST1 is responsible for mediating the sulfation of keratan in the cornea, which is important in maintaining corneal transparency.
In particular, CHST1 catalyzes the transfer of sulfate groups from 3’-phosphoadenosine 5’-phosphosulfate to position six of internal or terminal galactose residues (preferentially the residues adjacent to sulfated GlcNAc) on growing keratan sulfate chains.
CHST1 also contributes to the generation of L-selectin ligands.
Mutations in the CHST1 gene may play a role in macular corneal dystrophy (MCD).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CHST1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 126-171 of Human CHST1.
