The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway.
Choline kinase, the initial enzyme in the sequence, plays a role in cell growth proliferation.
A related protein, ChoKB (also, known as choline kinase beta), is a 395 amino acid enzyme that catalyzes the phosphorylation of choline by ATP in the presence of magnesium, thereby yielding phosphocholine and ADP.
Like all choline kinases, ChoKB possesses ethanoalamine kinase activity and catalyzes the phosphorylation of ethanolamine.
The gene encoding ChoKB is located less than 1 kb upstream of the CPT1B gene, suggesting that the ChoKB gene may regulate transcription CPT1B.
In mice, mutations and/or deletions in the gene encoding ChoKB are the cause of hindlimb muscular dystrophy and neonatal bone deformity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ChoKB. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-342 of Human ChoKB.
