Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA.
May also negatively regulate cell cycle progression during unperturbed cell cycles.
This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome.
Phosphorylation at Ser-280 by AKT1/PKB, may promote mono and/or diubiquitination.
Also phosphorylated at undefined residues during mitotic arrest, resulting in decreased activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Chk1, phosphorylated Ser280. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Serine 280.
