The EF-hand type Ca2+-binding proteins family consists of several members, including Centrin-1, Centrin-2 and Centrin-3.
The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation.
Tissues where cilia are present, such as the retina and testis, express both Centrin-1 and -2, but Centrin-2 is also expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), liver, skeletal muscle and cardiac muscle.
In these tissues, Centrin associates with the centrosomes, mitotic spindle poles and basal bodies.
Knockdown studies reveal a requirement for Centrin in centriole duplication and organization of spindle pole morphology and the completion of cytokinesis.
Centrin-3 plays a role in centrosome reproduction.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CETN2. Species Crossreactivity: Human, mouse, rat
