84-2312-97　［取扱停止］Cdc34 (Ubiquitin-conjugating Enzyme E2 R1, Ubiquitin-conjugating Enzyme E2-32kD Complementing, Ubiquitin-conjugating Enzyme E2-CDC34, Ubiquitin-Protein Ligase R1, UBCH3, UBE2R1, CDC34) 100ul　221590

特徴

• The cell division cycle (Cdc) genes are required at various points in the cell cycle.
• Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues.
• Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication.
• Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90.
• Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes.
• Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1/S phase transition.
• Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.