84-2312-95　［取扱停止］Cdc27 (Cell Division Cycle Protein 27 Homolog, Anaphase-promoting Complex Subunit 3, APC3, CDC27 Homolog, CDC27Hs, H-NUC, CDC27, ANAPC3, D0S1430E, D17S978E) 100ul　221589

特徴

• Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins.
• The cell division cycle (Cdc) genes are required at various points in the cell cycle.
• Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90.
• Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes.
• Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition.
• Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.