Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family.
Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC).
This complex consists of two proteins,Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle.
Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids.
The APC is thought to be stabilized by Cdc26 (also known as Scd26).
In addition to these APC proteins, Cdc5 is also required for completion of mitosis.
In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred..
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cdc20. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 80-130 of Human Cdc20.
