84-2312-49　［取扱停止］Cdc2 (Cyclin-Dependent Kinase 1, Cell Division Control Protein 2 Homolog, Cell Division Protein Kinase 1, p34 Protein Kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2) 100ul　221564

特徴

• Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase.
• Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation.
• Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161.
• In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15.
• Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161.
• Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.