The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine-rich extracellular domains.
Members of this family include FAS, OX40, CD27 and CD30.
Ligands for these receptors are often type II transmembrane glycoproteins, as is the case for CD27 and CD30.
CD27 is a homodimeric lymphocyte-specific surface antigen present on T and B lymphocytes.
Activation of the CD3 complex via the T cell receptor for antigen leads to an increase in CD27 expression.
Together, CD27 and its ligand, CD70, generate co-stimulatory signals required for complete T cell activation.
CD30 is a surface marker for neoplastic cells of the Hodgkin’s lymphoma and related hematologic malignancies.
CD30L has been shown to enhance the proliferation of the Hodgkin’s cell line HDLM-2, but exerts antiproliferative effects on large cell anaplastic lymphoma cell lines.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide
Specificity：Recognizes endogenous levels of human CD70.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 80-130 of human CD70.
