CD52 is a glycosylphosphatidylinositol (GPI)-linked surface antigen present at high levels on epithelial cells lining the male reproductive tract, thymocytes, lymphocytes, monocytes and macrophages.
It is also present at variable levels on lymphoid malignancies.
During sperm maturation, spermatozoa passing through the genital tract acquire CD52 that is shed from the epithelial cell lining into seminal plasma.
CD52 is detectable on the surface of epididymal sperm and in the ejaculate but not on spermatogenetic cells or testicular spermatozoa.
The peptide backbone of CD52, which consists of 12 amino acids, is considered a mere scaffold for posttranslational modifications, such as GPI-anchor and N-glycosylation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CD52. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human CD52.
