The B lymphocyte specific CD22 antigen, also designated B-lymphocyte cell adhesion molecule (BLCAM), sialic acid-binding Ig-like lectin 2 (Siglec-2) and Leu-14, is a type I integral membrane glycoprotein, structurally similar to other cell adhesion molecules (CAMs), which acts as a regulator of B cell signaling.
CD22 is expressed as both a cytoplasmic and membrane protein during discrete stages of B cell lymphocyte differentiation.
The cytoplasmic form of CD22, expressed early in B cell development, is a useful marker for acute lymphocytic leukemia.
The membrane form of CD22 is expressed in mature B cells prior to their differentiation into plasma cells.
Alternative splicing results in two different isoforms, CD22alpha and CD22beta.
The CD22beta monomer is the principally occurring isoform but CD22 also appears as a heterodimer of CD22beta and the shorter isoform, CD22alpha.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CD22. Species Crossreactivity: Human
