CD137, also designated ILA and 4-1BB in mouse, belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes.
CD137 is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages and dendritic cells.
Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells.
Crosslinking of the CD137 ligand induces apoptosis in resting lymphocytes.
In contrast, CD137 regulates peripheral monocyte survival by inducing a cytokine release profile, and is mediated by M-CSF and to a lesser extent by granulocyte-macrophage colony-stimulating factor and IL-3.
Soluble forms of CD137 are found in sera from patients with rheumatoid arthritis and may provide a negative control mechanism for immune responses.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CD137. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 80-130 of Human CD137.
