Human CD13 is expressed on the majority of peripheral blood monocytes and granulocytes.
It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines.
CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma.
CD13 is not expressed on B cells, T cells, platelets or erythrocytes.
In the mouse, CD13 is a non-covalently linked homodimer of ~150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.02% sodium azide, 50% glycerol. pH7.2.
Specificity:Recognizes endogenous levels of human CD13. Species Crossreactivity: mouse, rat.
Isotype:IgG
Calc Applications Abbrev:IF WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Full length recombinant protein corresponding to human CD13.