84-2309-53　［取扱停止］CBR1 (15 hydroxyprostaglandin Dehydrogenase [NADP+], 15-hydroxyprostaglandin Dehydrogenase [NADP+], Carbonyl Reductase [NADPH] 1, Carbonyl Reductase 1, CBR 1, CBR1, CBR1_HUMAN, CRN, NADPH Dependent carbonyl Reductase 1, NADPH-Dependent carbonyl Reductase 1, Prostaglandin 9 ketoReductase, Prostaglandin 9-ketoReductase, Prostaglandin E(2) 9 Reductase, Prostaglandin- ...　221412

特徴

• Carbonyl reductase is one of several monomeric,NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds.This enzyme is widely distributed in human tissues.
• Another carbonyl reductase gene,CRB3,lies close to this gene on chromosome 21q.
• NADPH-dependent reductase with broad substrate specificity.Catalyzes the reduction of a wide variety of carbonyl compounds including quinones,prostaglandins, menadione,plus various xenobiotics.Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol.
• Can convert prostaglandin E2 to prostaglandin F2-alpha.Can bind glutathione,which explains its higher affinity for glutathione-conjugated substrates.Catalyzes the reduction of S-nitrosoglutathione.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.