Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane.
On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis, and the compartmentalization of certain signaling molecules, including G protein-coupled receptors.
Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae.
Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino-terminal 31 amino acid sequence which is absent from the beta isoform.
Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level.
Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Caveolin-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 150-200 of Human Caveolin-1.
