The catenins, alpha, beta and y, are proteins which bind to the highly conserved,intracellular cytoplasmic tail of E-cadherin.
Together, the catenin/cadherincomplexes play an important role mediating cellular adhesion.
alpha-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin.
beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule.
beta-catenin has also been found in complexes with the tumor suppressor protein APC.
y-catenin, also known as plakoglobin, binds with alpha-catenin and N-cadherin.
It has been shown that the transmembrane phosphatase PTPμ associates with catenin/cadherin complexes and may regulate complex signaling.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Catenin-γ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 700-750 of Human Catenin-γ.
