beta-Catenin is a key downstream effector in the Wnt signaling pathway.
It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis.
CK1 phosphorylates beta-catenin at Ser45.
This phosphorylation event primes beta-catenin for subsequent phosphorylation by GSK-3beta.
GSK-3beta destabilizes beta-catenin by phosphorylating it at Ser33, Ser37, and Thr41.
Mutations at these sites result in the stabilization of beta-catenin protein levels and have been found in many tumor cell lines.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Catenin-beta, phosphorylated S37. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human Catenin-β around the phosphorylation site of Serine 37.
