alpha E-catenin (also designated alpha-catenin; cadherin-associated protein, alpha 1, 102kDa; and CAP102) plays a role in E-cadherin mediated cell-cell adhesion by linking E-cadherin to the cytoskeleton via beta- or y-catenin and Actin.
alpha E-catenin connects cell-density-dependent adherens junctions with the developmental hedgehog pathway and may provide a negative feedback loop controlling the size of developing cerebral cortex.
It is abundant in neuroepithelial precursor cells in the developing cortical ventricular zone of the brain, with reduced expression in the cortical plate.
alpha E-catenin-vinculin interactions play a role in the assembly of the apical junction complex in epithelia.
Catenins generally are thought to work as connectors that anchor E-cadherin to the cytoskeletal Actin bundle through the cadherin cytoplasmic domain.
Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, and is thus considered a contributing factor to metastasis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Catenin-alpha1/2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human Catenin-α1.
