a-catenins are a group of proteins associated with cadherin cell-cell adhesion molecules and play indispensable roles in the function of the cadherins.
a N-catenin is a linker between cadherin adhesion receptors and the Actin cytoskeleton and is essential for stabilizing dendritic spines in rodent hippocampal neurons in culture.
A deletion in this protein causes cerebellar and hippocampal lamination defects and impaired startle reaction.
a E- and a N-catenin appear to colocalize in cell bodies of neurons in dorsal root ganglia.
In mice, a N-catenin was found to occur at the roof plate of the mesencephalon and diencephalon, coinciding with Wnt-1 expression.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Catenin-alpha1/2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids C-terminus of Human Catenin-α2.
