Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli.
Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity).
Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adult tissue.
Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus.
Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini.
Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis.
Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30).
The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14).
As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Caspase-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide conjugated to KLH derived from within residues 150 - 200 of Human active Caspase 2.
