A proposed genetic marker of cardiac hypertrophy, CARP (cardiac ankyrin repeat protein, ANKRD1, Ankyrin repeat domain-containing protein 1, ) is a nuclear protein with an established role in regulation of cardiac gene expression.
A distinct increase in CARP expression occurs in rats with abdominal aorta constriction, spontaneous hypertension and Dahl salt-sensitivity.
In cardiomyocytes, CARP inhibits transcription of both cardiac troponin C and atrial natriuretic factor.
Specifically, expression of the CARP gene, which lies downstream of the cardiac homeobox gene Nkx2.5, inhibits Nkx2.5 transactivation of atrial natriuretic factor promoter.
An increase in CARP expression is observed in the ventricular tissue of patients with endstage heart failure.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CARP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 212-262 of Human CARP.
