Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation.
Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3.
Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus.
Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular diso4.
Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes.
Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of CAPN3. Species Crossreactivity: Human, mouse, rat
![CAPN3 (Calcium Activated neutral Proteinase 3, Calcium Activated neutral Proteinase 3, Calcium-Activated neutral Proteinase 3, calpain 3, (p94), Calpain L3, Calpain large Polypeptide L3, Calpain large Polypeptide L3, Calpain p94, calpain p94, large [Catalytic] Subunit, calpain, large Polypeptide L3, Calpain-3, CAN3_HUMAN, CANP 3, CANP3, CANPL3, CAPN 3, CAPN3, LGMD ...](https://aimg.as-1.co.jp/c/84/2307/07/85133520.jpg)