Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen.
Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum.
Calregulin has both low- and high-affinity calcium-binding sites.
Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins.
Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Calnexin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 540-590 of Human Calnexin.
