Spermatozoa gain fertilization capacitation and hyperactivation after residing in the uterus or oviduct.
Calcium, cAMP and protein tyrosine phosphorylation are necessary for the molecular mechanisms that allow for this transformation.
CABYR (Calcium-binding tyrosine phosphorylation-regulated protein), also known as Fibrousheathin-2 or Cancer/testis antigen 88, is a 493 amino acid protein that is expressed in sperm flagella and exhibits increased tyrosine phosphorylation during capacitation.
There are six named isoforms of CABYR that are produced as a result of alternative slicing events.
Specifically, isoform 1 is expressed in the testis, while isoform 3 and isoform 5 are expressed in pancreas, brain and various brain tumors.
Isoforms 1, 2 and 6 most likely bind calcium, whereas isoforms 3, 4 and 5 probably do not bind calcium.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of CABYR. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human CABYR.
