Cables, a 568 amino-acid protein, links Abl to cyclin-dependent kinase 5 (Cdk5).
Cables bound to Cdk5 functions as a substrate for phosphorylation by the Cdk5/p35 kinase.
Cables contains an area of weak homology to cyclin A and cyclin C.
In addition to its C-terminal Cdk5 binding domain, Cables also has six potential SH3 binding motifs (PXXP) clustered around the amino terminus, two of which are similar to motifs known to bind the Abl SH3 domain.
Cables forms a trimolecular complexwith Cdk5 and Abl in vivo.
All three proteins colocalize within cortical axons, particularly in their growth cones.
Cables and Abl may function as adaptor or scaffolding proteins to bind to Cdk5 and control its subcellular location in the neuron.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Cables1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 550-600 of Human Cables1.
