The complementcomponent proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactionsspecificto cells of hematopoieticlineage.
These proteins belong to thealpha2-macroglobulin family, but retain distinctive featuresincluding an anaphylatoxin domain and a netrin (NTR) domain.
The human C4 gene is polymorphic at two loci, C4A and C4B,mapping to chromosome 6p21.32.
C4Aexpressesthe Rodgers(Rg) blood group Ag, while C4B expressesthe Chido(Ch) blood group Ag.
C4 is expressed as a precursor that iscleaved into alpha, beta and y chains, all ofwhich are non-identicalcleavage products.
Thealphachain of C4may be cleaved to produce an acidicisotype, C4a,which reactswith amino groups, and a basicisotype, C4b,which reactswith hydroxyl groups.
Deficiency in the C4 gene is associatedwith autoimmune or immune complex disorders, such assystemiclupus erythematosus.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human C4A/C4B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human C4A.
