C/EBP beta is a member of the C/EBP transcription factor family.
The C/EBP beta gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites.
Initiation of translation at the in-frame AUGs forms four C/EBP beta isoforms.
C/EBP beta is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5).
C/EBP beta contributes to the regulation of the acute phase response in hepatocytes.
Stat3 has an important function in IL-6-mediated transcription of the C/EBP beta gene that has direct implication for acute phase response in liver cells.The 35kDa C/EBP beta form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP beta isoforms during acute-phase reaction occurs through its upregulation and structural modification.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of C/EBP-beta, phosphorylated T235/188. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human C/EBP-β around the phosphorylation site of Threonine 235.
