C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine.
C/EBP alpha is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas.
C/EBP alpha uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells.
In the liver, C/EBP alpha is a transactivator of several genes, which are regulated by growth hormone.
Growth hormone enhances not only the levels of C/EBP alpha mRNA and protein, but also the DNA-binding activity of C/EBP alpha.
C/EBP alpha functions as an important transcription factor that regulates different genes, including Prolactin gene expression.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of C/EBP alpha, phosphorylated S21. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human C/EBP α around the phosphorylation site of Serine 21.
