C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine.
C/EBP alpha uses a bipartite structural motif to bind DNA.
Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper.
Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces.
C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells.
Additional family members include C/EBP beta, C/EBP y, C/EBP delta and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha.
Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of C/EBP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 188-245 of Human C/EBP-ε.
