84-2305-23　［取扱停止］c-TAK1 (C-TAK1, Cdc25C Associated Protein Kinase 1, Cdc25C-Associated Protein Kinase 1, cTAK1, ELKL Motif Kinase 2, EMK-2, Emk2, ETK 1, KIAA4230, KP78, MAP microtubule affinity Regulating Kinase 3, MAP/microtubule affinity-Regulating Kinase 3, Mark3, MARK3, Par 1a, PAR1A, Protein Kinase STK10, Ser/Thr Protein Kinase PAR-1, Serine threonine Protein Kinase p78, Seri ...　221195

特徴

• c-TAK1 (Cdc25C associated protein kinase) phosphorylates Cdc25C on Ser 216 and is ubiquitously expressed in various human tissue and cell lines.
• C-TAK1 is distinct from Chk1, which also phosphorylates Cdc25C on Ser 216 in response to DNA damage.
• Phosphorylation of Cdc25C allows for the preferential binding of 14-3-3 proteins, subsequently retaining Cdc25C in the cytoplasm.
• Thus, the binding of 14-3-3 proteins prevents Cdc25C from dephosphorylating Cdc2 in the nucleus, thereby controlling the entry of the cells into mitosis.
• It is suggested that C-TAK1 mediates the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.