c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene.
v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic.
c-Rel has been shown to represent a constituent of the κB site binding transcription factor NFκB, which plays a crucial role in the expression of immunoglobulin κ light chain gene.
In contrast to c-Rel, v-Rel is truncated in its C-terminal transactivation domain and does not appear to function as a transcriptional transactivator.
It has thus been postulated that v-Rel may interfere with the normal transcription of NFκB regulated genes and thus cause transformation by a mechanism analogous to v-ErbA, which binds to the thyroid hormoneresponsive region in certain erythroid genes needed for differentiation, but cannot be activated by thyroid hormone.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of c-Rel, phosphorylated S503. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.
