The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus.
The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes.
In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family.
Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes.
In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun.
Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.
Human c-Fos may be phosphorylated at Thr 232.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of c-Fos. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1-50 of Human c-Fos.
