BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC).
It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1.
BUB3 is essential for the kinetochore localization of BUB1 and BUBR1.
As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis.
The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle.
This acts to ensure accurate chromosome segregation.
In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.
BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of BUB3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 251-300 of Human BUB3.
