TFIID is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element.
It is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs).
Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription.
The largest subunit (TAF) of TFIID is a protein designated TAF II p250.
B-TFIID is an initial factor composed of TBP and TAF II p170 that has been identified as a pol II transcription factor.
TAF II p170 has been shown to have potent (d)ATPase activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of BTAF1. Species Crossreactivity: Human, mouse, rat
