The Brahma protein (Brm) is an ATPase subunit of the Drosophila melanogaster Brm complex, which is highly related to the mammalian SWI/SNF chromatin-remodeling complex.
Brm is a transcriptional activator of Hox genes and associates with nearly all transcriptionally active chromatin in a pattern that is non-overlapping with that of Polycomb, a repressor of Hox gene transcription.
The Brm complex is an essential coactivator for the trithorax group protein Zeste, a DNA-binding activator of homeotic genes.
Reduction of Brm function dramatically reduces the association of RNA polymerase II with Drosophila salivary gland chromosomes, suggesting that the chromatin remodeling activity of the Brm complex plays a general role in facilitating transcription by RNA polymerase II.
Brm acts as a dominant suppressor of the rough eye phenotype that results from a hypomorphic mutation of Drosophila cyclin E by inhibiting S phase entry by acting downstream of cyclin E protein accumulation.
The interaction of the Brm complex with chromatin may be modulated by BAP111, which is highly associated with the Brm complex in Drosophila embryos via an HMG domain.
Brm is highly expressed in unfertilized eggs and early embryos.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human BRM. Species Crossreactivity: mouse, and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1387-1432 of human BRM.
