BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor.
It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex).
BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex.
This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination.
Mutations in the BRCA1 gene are responsible for ~40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers.
Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of BRCA1, phosphorylated S1423. Species Crossreactivity: Human, rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic phosphopeptide derived from human BRCA1 around the phosphorylation site of Serine 1423.
