The zinc finger protein, basonuclin, is a putative rDNA transcription factor with highly restricted tissue distribution.
Basonuclin is abundantly expressed in keratinocytes of the basal layer of the epidermis, the outer sheath of hair follicles and in the germ cells of the testis and ovary.
Although basonuclin is associated with chromatin throughout the cell cycle, including mitosis, it disappears when cells become post-mitotic.
In the epidermis, basonuclin, which is mainly localized to the cytoplasm, translocates to basal cell nuclei during different stages of keratinocyte growth.
Basonuclin may enhance rRNA synthesis by elevating transcription from an rDNA promoter and inhibiting RNA polymerase I transcription through its zinc finger domain.
Therefore, basonuclin may be a cell-type-specific transcription factor for rDNA transcription.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of BNC1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human BNC1.
