84-2302-72　［取扱停止］BMF (Bcl 2 modifying Factor, Bcl-2-modifying Factor, Bcl2 modifying Factor, Bmf, BMF_HUMAN, FLJ00065) 50ul　221066

特徴

• The BH3-only proteins, which include Blk, Bad, Bik, Hrk, BID, Bim, NOXA, PUMA and Bmf, are proapoptotic members of the Bcl-2 family.
• Bcl-2 modifying factor (Bmf) is a BH3-only protein that binds prosurvival Bcl-2 family members to initiate apoptosis.
• Bmf is sequestered to Myosin V motors on Actin in the cytoskeleton by associating with Dynein light chain 2 (DLC2) homodimers.
• If the cell undergoes loss of attachment (anoikis), the cytoskeleton is disrupted and Bmf is released from DLC2.
• Bmf then translocates to the mitochondria, where Bcl-2 (an anti-apoptotic family member) is sequestered.
• The BH3 domain of Bmf facilitates binding to a hydrophobic groove on the surface of Bcl-2.
• Binding results in a caspase cascade leading to apoptosis.
• Bmf is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.