The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas.
Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal.
A protein designated Bax p21 (i.e., Bcl-associated X protein) has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2.
Overexpression of Bax accelerates apoptotic death.
Natural born killer (NBK), also known as Bik, is a protein that is functionally related to Bax, although the two proteins share very little sequence homology.
NBK does not contain the conserved Bcl-2 homology domains (BH domains) characteristic of the Bcl-2 family.
It does however, share nine amino acids with Bax in a region designated BH3, which may be the critical determinant for the protein’s death-promoting activities.
Phosphorylation of NBK at Ser35 and Thr33 is required for eliciting efficient apoptotic activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of BIK/ NBK, phosphorylated T33. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from human BIK/ NBK around the phosphorylation site of Threonine 33.
