The Bcl-2 family of proteins plays a central regulatory role in apoptosis.
Bcl-rambo, a member of the Bcl-2 family, localizes to the mitochondria and, like other Bcl-2 family members, contains all four BH domains.
Although Bcl-rambo shares structural similarity to other Bcl-2 members, it differs from them in its unique C-terminal region.
Bcl-rambo has a 250 amino acid sequence containing two tandem repeats that preceeds the membrane anchor region at its C-terminus.
Additionally, it is the membrane anchor C-terminal region of Bcl-rambo, not its Bcl-2 homology motifs, that is responsible for its pro-apoptotic activity.
Bcl-rambo induces apoptosis when overexpressed and appears to do so by promoting mitochondrial cyto-chrome c release.
It may also facilitate the activation of caspase-3.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of BCL2L13. Species Crossreactivity: Human, mouse
