Brain-specific angiogenesis inhibitors, including BAI-1, BAI-2 and BAI-3, are integral membrane proteins belonging to the G protein-coupled receptor 2 family.
In addition to inhibiting angiogenesis in the brain, BAI proteins are also expressed in the heart, thymus, skeletal muscle, and a variety of cell lines.
BAI-1 protein is specifically expressed in the brain and found to localize to the cytoplasm and membrane in neuronal cells of the cerebral cortex.
Reduced expression of BAI-1 in some glioblastoma cell lines and cancer tissues implicates the functional role of BAI-1 as an inhibitor of angiogenesis.
The exact mechanisms underlying BAI-1 anti-angiogenetic activity are still being investigated.
BAI-1 may be involved in mediating the p53 signal in suppression of glioblastoma, as well as in cell adhesion and signal transduction.
Additional research shows an inverse correlation with vascularization and BAI-1 expression in both colorectal carcinomas and pulmonary adenocarcinomas.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of BAI-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 711-760 of Human BAI-1.
