Bag-5 (Bcl-2-associated athanogene 5), also known as Bag family molecular chaperone regulator 5, is a member of the Bag family of proteins and contains four Bag domains.
Via their Bag domain, Bag proteins bind with high affinity to the HSC 70/HSP 70 ATPase domain, regulating chaperone activity and apoptosis.
Bag-5 is a component of the HSP 70/Parkin complex and acts to inhibit Parkin E3 ubiquitin ligase activity and HSP 70 chaperone activity.
In this complex, Bag-5 directly interacts with the ATPase domain of HSP 70 and the N-terminal linker region of Parkin.
Bag-5 expression is induced upon dopaminergic neuron injury and functions to sensitize the neurons to injuryinduced cell death.
In addition, Bag-5 may be a useful target in therapies for neurodegenerative diseases such as Parkinson’s disease which is caused by a mutation in the gene encoding Parkin.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of BAG5. Species Crossreactivity: Human, mouse
