The cytokine TNF (tumor necrosis factor) signals through the TNF-R1 receptor to activate various cellular pathways, including apoptosis and NFkB activation.
TNF binding induces receptor aggregation, resulting in the recruitment of TRADD, FADD, TRAF2 and RIP to the intracellular “death” domain of the receptor complex, which in turn activates signaling pathways including apoptosis and NFkB activation.
SODD, for silencer of death domains, was found to be associated with the intracellular “death” domain of TNF-R1 in the absence of TNF stimulation.
TNF treatment results in the release of SODD from TNF-R1, allowing the recruitment of TRADD and TRAF2 to the receptor complex.
Thus, SODD may play a role in preventing spontaneous signaling by death-domain receptors, in the absence of ligand.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Bag-4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 411-460 of Human Bag-4.
