c-Myb is the cellular homolog of the leukemogenic avian retroviral protein v-Myc.
c-Myb is expressed predominantly in immature and rapidly dividing hematopoietic cells, and cellular levels of c-Myb substantially decreases as cells reach terminal differentiation.
B-Myb is expressed in a wide variety of proliferating cells, with levels accumulating during the G1 to S phase transition.
A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity:Recognizes endogenous levels of B-Myb. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:IF WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to amino acids 543-590 of Human B-Myb.