Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds.
ATPases contain two linked complexes, F1, the hydrophilic catalytic core, and F0, the membrane-embedded protein channel.
F1 consists of three alpha chains and three beta chains, which are weakly homologous, as well as one y chain, one delta chain and one ε chain.
F0 consists of three subunits, a, b and c.
The alpha chain of F1 is a regulatory subunit that contains 509 amino acids.
Mitochondrial ATPase alpha chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of ATP5A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 210-260 of Human ATP5A.
